• Dr. Francesc Broto Puig

Dr. Francesc Broto Puig
Sección de Cromatografía / Departamento de Química Analítica.
Analytical Chemistry Group (QuAn)
IQS School of Engineering
Universitat Ramon Llull
Via Augusta 390, 08017 Barcelona
Tel. +34 93 2672000
Fax +34 93 2056266

francesc.broto@iqs.url. edu

Ph.D. in Chemistry, Universitat Ramon Llull (1993, URL)
Chemical Engineering of IQS (1987 (1980, IQS)
Degree in Chemistry (1992, URL)

Professor (2009- )


Chemistry (Degree in Chemistry and Degree in Chemical Engineering)
Instrumental Analysis (Degree in Chemistry)


Research Lines

  • Study of instrumental chromatographic and related chromatographic techniques: GC, LC, HPCE, GC-MS, LC-MS, MS. Application to the environment, indoor atmospheres, food, and drugs.
  • Characterization of dietary fats: development of analytical methods for the determination of omega-3, omega-6 and trans fatty acids. Study of its presence and evolution in the preparation of different types of food.
  • Study of organic pollutants in the atmosphere (emissions and immissions): ozone precursors, siloxanes and PAHs. Development of analytical methods for its determination. Study of the sources and evolution of these pollutants in the air.
  • Study of organic pollutants in the interior atmosphere of a museum (emissions and immissions): acids and acid precursors. Development of analytical methods for its determination. Study of the sources and evolution of these pollutants.
  • Analysis of drugs and related substances by chromatographic and electrophoretic techniques. Design, development, validation, and transfer of procedures
  • Chromatographic retention /structure relationship.


Representative Publications

Singla M., Díaz J., Broto-Puig F., Borrós S. Sorption and release process of polybrominated diphenyl ethers (PBDEs) from different composition microplastics in aqueous medium: Solubility parameter approach. Environ. Pollut. 262 (2020) 114377. https://doi.org/10.1016/j.envpol.2020.114377

Raich-Montiu, J.; Ribas-Font, C.; Arespacochaga, N.; Roig-Torres, E.; Broto-Puig, F.; Crest, M.; Bouchy, L.; Cortina, J. L., Analytical methodology for sampling and analysing eight siloxanes and trimethylsilanol in biogas from different wastewater treatment plants in Europe, Analytica Chimica Acta 812 (2014) 83-91.
Cirera-Domènech E.; Estrada-Tejedor R.; Broto-Puig F.; Teixidó J.; Gassiot-Matas M.; Comellas Ll.; Lliberia J.Ll.; Méndez A.; Paz-Estivill S.; Delgado-Ortiz M.R., Quantitative structure-retention relationships applied to liquid chromatography gradient elution method for the determination of carbonyl-2,4-dinitrophenylhydrazone compounds, Journal of Chromatography A 1276 (2013) 65-77.
Pereira T.S.; Beltrami L.S.; Rocha J.A.V.; Broto P F.; Comellas Ll.; Salvadori MF; Vargas V.M.F, Toxicogenetic monitoring in urban cities exposed to differentairborne contaminants, Ecotoxicology and Environmental Safety 90 (2013) 174-182.
Alfaro C.V.; Broto-Puig F.; Agut M.; Comellas Ll., Estudio de la producción de aflatoxinas B1, G1, B2 y G2 en semillas de anacardo por parte de Aspergillus parasiticus CECT 2681 mediante cromatografía líquida de ultra-alta resolución, Afinidad 70 (2013) 170-174.
Estrada-Tejedor R.; Sabaté N.; Broto F.; Nonell S.
Octanol-water partition coefficients of highly hydrophobic photodynamic therapy drugs: a computational study, Afinidad 70 (2013) 250-256.
Quintela M.; Báguena J.; Gotor G.; Blanco Mª J.; Broto F., Do we agree with chromatograph operational qualification provided from manufacturers? Afinidad 70 (2013) 6-12.
Quintela M., Báguena J., Gotor G., Blanco M J., Broto F., Estimation of the uncertainty associated with the results based on the validation of chromatographic analysis procedures: Application to the determination of chlorides by high performance liquid chromatography and of fatty acids by high resolution gas chromatography, Journal of Chromatography A, 1223 (2012) 107-117.
Pereira, T.S.; Gotor, G.N.; Beltrami, L.S.; Nolla, C. G.; Rocha, J.A. V.; Broto, F.; Comellas, Ll.; Vargas, V.M. F., Salmonella mutagenicity assessment of airborne particulate matter collected from urban areas of Rio Grande do Sul State, Brazil, differing in anthropogenic influences and polycyclic aromatic hydrocarbon levels, Mutation Research-Genetic Toxicology and Environmental Mutagenesis 702 (2010) 78-85.
Navajas H.; Broto-Puig F.; Agut M.; Comellas L., Análisis simultáneo de aflatoxinas y ocratoxina A en compost por HPLC-MS. Afinidad, 56 (2010) 94-99.
Ortiz X, Marti R, Montaña MJ, Gasser M, Margarit L, Broto F, Díaz-Ferrero J. Fractionation of persistent organic pollutants in fish oil by high performance liquid chromatography equipped with a 2-(1-pyrenyl)ethyl silica column, Analytical and Bioanalytical Chemistry, 398 (2010) 985-994.

Quintela, M.; Báguena, J.; Broto, F.; Margarit, L.; Blanco, M.J.; Optimización y revalidación del análisis de nitrógeno por el método Kjeldahl en muestras de taurina, Afinidad, 66 (2009), 200-205.

Mejia, G.; Nadal, R.; Bago, B.; Broto, F.; Comellas, Ll., Thiosteroids in sewage sludge and its post-treatment products, International Journal of Environmental Analytical Chemistry 88 (2008) 803-812.

Báguena, J.; Gotor, G.; Broto, F.; Blanco, M.J.; Cálculo de la incertidumbre asociada a los resultados basado en la validación de un procedimiento de análisis. Aplicación en la determinación de cloruros por HPLC en lixiviados, Afinidad, 65 (2008), 11-19.

Ventura, M.; Guillén, D.; Anaya, I.; Broto-Puig, F.; Lliberia, J.L; Agut M.; Comellas Ll.;Ultra-performance liquid chromatography/tandem mass spectrometry for the simultaneous analysis of aflatoxins B1, G1, B2, G2 and ochratoxin A in beer, Rapid Communications in Mass Spectrometry, 20 (2006) 3199- 3204.

M.Ventura, I. Anaya, F. Broto-Puig, M. Agut, L. Comellas, Two dimensional thin layer chromatographic method for the analysis of ochratoxin A in green coffee, Journal of Food Protection, 68, 9, (2005) 1920-922.

M. Gassiot, L. Comellas, F. Broto-Puig, D. Zandio, G. Mejía, Desarrollo de nuevos métodos para el análisis de Doxiciclina en residuos orgánicos por HPLC. Afinidad, 62 (2005), 415- 421

L.Comellas, M. Agut, F. Broto-Puig, J. Díaz-Ferrero, M. Belío, B. Bagó, G. Mejía, Análisis por cromatografía ultrarrápida de los alquilbencenosulfonatos lineales (LAS) en fangos de depuradora y estudio de su evolución durante los procesos de post-tratamiento, Afinidad, 62 (2005), 428-432

L. Comellas, B. Bagó, Y. Martin, G. Mejía, F. Broto-Puig, J. Díaz-Ferrero, M.Agut, Di-(2-ethylhexyl)phthalate in sewage sludge and post-treated sludge: thermally dried sludge: Quantitative determination by HRGC-MS and mass spectral characterization, Chemosphere, 59 (2005), 1191-1195.

B. Bagó, Y. Martin, G. Mejía, F. Broto-Puig, J. Díaz-Ferrero, M. Agut, L. Comellas, Di-(2-ethyl)phtalate in sewage sludge and post-treated sludge: Quantitative determination by HRGC-MS and mass spectral characterization, Chemosphere 59 (2005), 1191-1195.

M. Ventura, A. Gómez, I. Anaya, J. Díaz, F. Broto, M. Agut, L. Comellas, Determination of aflatoxins B1, G1, B2 and G2 in medicinal herbs by liquid chromatography tandem mass spectrometry, Journal of Chromatography A, 1048 (2004) 25-29.
Ventura, M; Vallejo, C.; Anaya, I.; Broto, F.. Agut, M.; Comellas, L., Analysis of Ochratoxin A in coffee by solid-phase clean-up and narrow-bore liquid chromatography-fluorescence detector-mass spectrometry, Journal of Agricultural and Food Chemistry, 51 (2003) 7564-7567.
Ventura, M; Agut, M.; Broto-Puig, F.; Comellas, L., Determination of ochratoxin A using solid-phase extraction and narrow-bore HPLC with fluorescence and ESI-MS detector, Afinidad, 60 (2003) 382-386.
E. Pujadas, J. Díaz-Ferrero, R. Martí, F. Broto-Puig, L. Comellas, M.C. Rodríguez-Larena, Application of the new C18 Speedisks to the analysis of polychlorinated dibenzo-p-dioxins and dibenzofurans in water and effluent samples, Chemosphere, 43 (2001) 449-454.
Molina, L.; Díaz-Ferrero, J.; Coll, M.; Martí, R.; Broto-Puig, F.; Comellas, L.; Rodríguez-Larena, M.C., Study of evolution of PCDD/F in sewage sludge-amended soils for land restoration purposes, Chemosphere, 40 (2000) 1173-1178.
Martínez, M.; Díaz-Ferrero, J.; Martí, R.; Broto-Puig, F.; Comellas, L.; Rodríguez-Larena, M.C., Analysis of dioxin-like compounds in vegetation and soil samples burned in Catalan forest fires. Comparison with the corresponding unburned material, Chemosphere, 41 (2000) 1927-1935.
L. Molina, J. Díaz-Ferrero, R. Marti, F. Broto-Puig, L. Comellas, M.C. Rodríguez-Larena, Development and Validation of a Method for Analyzing Polychlorinated Dibenzo-p-Dioxins and Polycholorinated Dibenzofurans in Seawage Sludge Samples, Chromatographia, 52 (2000) 87-92.
L. Molina, M. Cabes, J. Díaz-Ferrero, M. Coll, R. Marti, F. Broto, L. Comellas, M.C. Rodríguez-Larena, Separation of non-orto polychlorinated biphenyls congeneres on pre-packed carbon tubes. Application to analysis in sewage sludge and soil samples, Chemosphere, 40 (2000) 921-927.
M. Martínez-Cored, E. Pujades, J.Díaz-Ferrero, M. Coll, R. Martí, F. Broto-Puig, L. Comellas, M. C. Rodríguez-Lerena, Fractionation of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and planar polychlorinated biphenyls by high performance chromatography on a pyrenyl-silica column, Fresenius Journal of Analytical Chemistry, 364 (1999) 576-583.
S. Rodríguez, C. Masalles, N. Agulló, S. Borrós, L. Comellas y F. Broto, Identification of the Intermediates Sulfur Vulcanitation of Natural Rubber, Kautschuk Gummi Kunststoffe, 52 (1999) 438-445.
X. Batllori, F. Broto-Puig, M.C. Rodríguez-Larena, La Conferencia sobre el Cambio Climático celebrada en Kioto. El Efecto Invernadero y el Calentamiento Global, Afinidad, 474 (1998) 85-92.
E. Mangas, M.T. Vaquero, L. Comellas y F. Broto-Puig, Analysis and fate of aliphatic hydrocarbons, linear alkylbencenes, polychlorinated biphenyls and polyciclic aromatic hydrocarbons in sewage sludge-amended soils, Chemosphere,36 (1998) 61-72.
N. Tharsis, J.L. Portillo, F. Broto-Puig y L. Comellas, Simplified reversed-phase conditions for the determination of benzimidazole fungicides in fruits by HPLC with UV detection, Journal of Chromatography A, 778 (1997) 95-101.
A. Sabala, J.L. Portillo, F. Broto-Puig y L. Comellas, Developement of a new high-performance liquid cromatography method to analyse N-methylcarbamate insecticides by a simple post-column derivation system and flurescence detection, Journal of Chromatography A, 778 (1997) 103-110.
Folch, I.; Vaquero, M. T.; Comellas, L.; Broto-Puig, F., Extraction and clean-up methods for improvement of the chromatographic determination of polychlorinated biphenyls in sewage sludge-amended soils: elimination of lipids and sulfur, Journal of Chromatography A, 719 (1996) 121-130.
M.E.Barrio, J.LL.Lliberia, Ll.Comellas, F.Broto-Puig, Pyrolysis-gas chromatography applied to the study of organic matter evolution in sewage sludge-amended soils using nitrogen-phosphorus, flame ionozation and mass spectrometric detection, Journal of Chromatography A, 719 (1996) 131-139.
J.Díaz, J.Ll.Lliberia, Ll.Comellas, F.Broto-Puig, Amino Acid and amino Sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate by high-performance liquid chromatography and fluorescence detection, Journal of Chromatography A, 719 (1996) 171-179.
Sape, H.; Lliberia, J.Li; Comellas, L.; Broto-Puig, F, Determination of volatile N-nitrosamines and nitrosated compounds in extracts of artificial saliva from nipples, dummnies and other rubber derivates, Afinidad, 53 (1996) 399-403.
Barrio, M. Eugenia; Broto-Puig, F.; Comellas, Lluis; Lliberia, Josep Li.; Pich, Marta; Bonmati, Manel, Application of new pyrolysis indexes to the study of the evolution of organic matter in soils amended with sewage sludge, Afinidad, 53  (1996) 171-175.
Stronguilo M.L., Vaquero M.T., Comellas L. Broto-Puig F., The fate of petroleum aliphatic hydrocarbons in sewage sludge amended soils, Chemosphere, 66 (1994) 21-29.
Codina G., Vaquero T., Comellas L., Broto-Puig F, Comparison of various extraction and clean-up methods for the determination of polycyclic aromatic hydrocarbons in sewage sludge-amended soils, Journal of Chromatography A, 673 (1994) 21-29.
L, A. Díaz, T. Moreno, F. Broto-Puig, Determinación de nipradilol en plasma mediante HPLC, Afinidad, 50 (1993) 407-410.
F. Broto, M. Zapata, L. Comellas y M. Gassiot, Aplicación de un microreactor de hidróxido potásico «on-line» en la determinación de compuestos organoclorados de alto peso molecular por cromatografía de gases con columnas capilares, Afinidad, 45 (1988) 9-18.
A. Díaz, L. Comellas, M.Gassiot, y F. Broto, Determinación de gentamicina en plasma mediante cromatografía líquida de alta eficacia en fase reversa, Afinidad, 45 (1988) 443-446.
M. Aceves, J. Grimalt, J. Albaigés, F. Broto, L. Comellas y M. Gassiot, Analysis of hydrocarbons in aquatic sediments. II. Evaluation of common prepatative procedures for petroleum and chlorinated hydrocarbons, Journal of Chromatography A, 436 (1988) 503-509.


Research Groups

Analytical Chemistry Group (QuAn)