• Trabaja con nosotros | Pre-Doctoral Fellowship/Contract (FPI)


The Laboratory of Biochemistry, Bioengineering Department at the Institut Químic de Sarrià, University Ramon Llull in Barcelona offers a PRE-DOCTORAL FELLOWSHIP / CONTRACT (FPI) from the Plan Estatal de Investigación Científica y Técnica y de Innovación del Ministerio de Ciencia e Innovación to undertake a PhD thesis in the framework of the funded project: PID2019-104350RB-I00. PROTEIN ENGINEERING AND METABOLIC ENGINEE-RING OF GLYCOZYMES FOR THE PRODUCTION OF BIOACTIVE GLYCANS AND GLYCOCONJUGATES TARGETING BIOMEDICAL APPLICATIONS. (GLYCODESIGN)

Project Abstract

Carbohydrate Active Enzymes (CAZYmes) are a ubiquitous class of enzymes that catalyze the biosynthesis, breakdown and transformation of glycan structures. They are a paradigm with regard to substrate recognition and specificity owing to the large diversity of glycan and glycoconjugate structures in nature. They are highly selective and able to recognize subtle differences in the sequence and stereochemistry of their carbohydrate substrates. The struc-tural information encoded by glycans is particularly apparent in molecular recognition events such as cell-cell interactions, pathogen-host interactions and signaling events.

The purpose of the project is to understand and modify substrate specificity of target CAZymes. Through protein engineering of CAZYmes and metabolic engineering using CAZYmes, the goal is to produce targeted, high-value glycans and glycoconjugates for applications in biotechnology and biomedicine.

The PhD project in the field of GLYCOBIOLOGY will focus on the discovery, characteriza-tion and engineering of CAZYmes (such as deacetylases, glycosidases and glycosyltransfer-ases) involved in bacterial and fungal cell wall metabolism with a dual objective: a) unravel structure-function relationships to identify key enzymes as therapeutic targets against patho-genic microorganisms, b) engineer substrate specificity (rational design and directed evolu-tion approaches) to develop biocatalysts for programmed chemoenzymatic synthesis of gly-co-conjugates.

Candidate's Profile

  • Graduate in Biochemistry, Biotechnology, Biology or Chemistry.
  • Master in Biochemistry, Biotechnology or related areas.
  • Experience in molecular biology and / or protein biochemistry during an internship in a company, university or research institution or collaboration in a research group.

Functions

  • Join a multidisciplinary research group composed of chemists, chemical engineers, bio-chemists and biotechnologists to carry out a doctoral thesis in a collaborative project with other team members, and enroll the PhD Program in Bioengineering by the University Ra-mon Llull.
  • The project involves the use of interdisciplinary techniques of molecular biology, expression and purification of proteins, chemical biology, biochemical and biophysical techniques, bioin-formatics and molecular modeling.

How to apply?

If you are interested in doing a doctoral thesis, motivated for research, with the ability to work in a dynamic team, send your detailed curriculum vitae, academic record and cover letter detailing your experience in laboratory techniques before October 15, 2020 by e-mail to antoni.planas@iqs.es in order to apply to the FPI call from the Ministerio de Ciencia e Innovación, where submission will be accepted from October 13th to October 27th, 2020.

You will find further information and application forms here

 

Apply now