• IQS acquires a QTOF X500B

Friday, 18 December, 2020
IQS Tech Transfer


The QTOF X500B device installed in the IQS laboratories

IQS has acquired a QTOF X500B device from SCIEX to support advanced research and technology transfer and to provide industry services, thereby expanding its range of high-end scientific equipment.

The university acquired a QTOF X500B connected to an Exion LCAD chromatograph. This hybrid quadrupole LC/MS/Ms mass spectrometer with time of flight (QTOF) features a range of m/z masses from 5 to 2250 m/z in quadrupole mode and up to 40 kDa in QTOF mode.

This device has comparable sensitivity to a high-performance triple quadrupole mass spectrometer that can work at very high scanning speeds, without compromising the resolution and with optimal mass accuracy.

Due to this design, the device can work in SWATH™ mode, an exclusive acquisition mode by SCIEX for QTIS devices that can obtain the mass spectrum and the spectrum of all the fragments of the specimens present in a sample, thereby attaining a digital record of the whole.

All this allows researchers to obtain a complete impartial MS and MS/MS registry of any sample with known or unknown components. The device can thus be used both for quantitative as well as qualitative work flows.

QTOF X500B applications

Thanks to its versatility and capacities, this device can be used for different applications, including:

• Identifying impurities, through libraries such as Chemspider.
• Characterising biological medicines.
• Determining intact mass. This device is capable of determining the molecular weight of a large protein through the spectra acquired from the mass.
• Establishing a drug-antibody ratio. The device is capable of verifying the drug load of the drug-antibody ratio and its distribution throughout the sample through reconstructed spectra.
• Verifying peptide mass.

This tool also makes it possible to work in MRMHR mode to function as QQQ with additional functions, allowing the instrument to be used for any determination, together with other instruments already in the laboratory.


This device can be used in a wide range of industrial sectors, including the following notable applications:

Pharmaceutical sector: bio-analysis, Discovery, QC, Pk, ADME, metabolism.
Food sector: detection of pesticides, mycotoxins, banned pollutants, multiresidue, sugars, lipids, proteins, and cases of fraud.
Environmental sector: water legislation, PFAs, haloacetics, endocrine disruptors, polar pesticides.
Clinical sector: immunosuppressants, neonatal screening, toxicology, antivirals, antidoping, amino acids, nucleotides.
Chemical sector: contact materials, detergents, cosmetics, phthalates, paint pigments.
Other applications in biopharma, proteomics, genomics, metabonomics, biotechnology, and nanotechnology.